Electron microscopy of beef heart mitochondrial F1 -ATPase
نویسندگان
چکیده
منابع مشابه
Structure of mitochondrial F1-ATPase studied by electron microscopy and image processing.
The structure of soluble F1-ATPase (EC 3.6.1.3) has been investigated by computer analysis of individual molecular images extracted from electron micrographs of negatively stained particles. A total of 1241 images was interactively selected from several digitized micrographs and these images were subsequently aligned relative to different reference images. They were then submitted to a multivar...
متن کاملNucleotide binding sites on beef heart mitochondrial F1-ATPase. Cooperative interactions between sites and specificity of noncatalytic sites.
We have studied the properties of beef heart mitochondrial F1 having inhibitory MgADP bound at one of the three catalytic sites and various levels of occupancy of the three noncatalytic nucleotide sites including zero, two, or three ADP/ATPs or two ADP/ATP plus one GTP. The properties examined include the rate of MgATP-dependent reactivation and the rate of increase in the fraction of F1 contai...
متن کاملCa21 activation of heart mitochondrial oxidative phosphorylation: role of the F0/F1-ATPase
Territo, Paul R., Vamsi K. Mootha, Stephanie A. French, and Robert S. Balaban. Ca21 activation of heart mitochondrial oxidative phosphorylation: role of the F0/F1ATPase. Am. J. Physiol. Cell Physiol. 278: C423–C435, 2000.—Ca21 has been postulated as a cytosolic second messenger in the regulation of cardiac oxidative phosphorylation. This hypothesis draws support from the well-known effects of C...
متن کاملStructure of the mitochondrial F1 ATPase at 9-A resolution.
The soluble portion (F1 ATPase) of the mitochondrial ATP-synthesizing system is a multisubunit enzyme of molecular weight 380,000. It is composed of five different subunits, alpha, beta, gamma, and epsilon. The subunit stoichiometry is not known but there are strong suggestions that it is alpha 3 beta 3 gamma delta epsilon. We have determined the three-dimensional structure of the F1 ATPase of ...
متن کاملStructural preferences for the binding of chromium nucleotides by beef heart mitochondrial ATPase.
The mono- and bidentate forms of adenosine 5'-diphosphate, chromium (III) salt (CrADP) were separated using Sephadex G-10 column chromatography. The isomeric purity of the two forms was monitored using high voltage electrophoresis and column chromatography. The same techniques were employed to assess the purity of the mono-, bi-, and tridentate forms of adenosine 5'-triphosphate, chromium (III)...
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ژورنال
عنوان ژورنال: FEBS Letters
سال: 1984
ISSN: 0014-5793
DOI: 10.1016/0014-5793(84)80144-1